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polystyrene round-bottom tube with cell strainer cap  (Corning Life Sciences)

 
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    Structured Review

    Corning Life Sciences polystyrene round-bottom tube with cell strainer cap
    Polystyrene Round Bottom Tube With Cell Strainer Cap, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/round+bottom+polystyrene+tube/cell+strainer+tube/pmc12274921-57-2-10
    Average 90 stars, based on 1 article reviews
    polystyrene round-bottom tube with cell strainer cap - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Incubation:

    Article Title: Regulation of hyperoxia-induced neonatal lung injury via post-translational cysteine redox modifications.
    Article Snippet: .. The minced tissue of each sample was collected into their own 5 mL round bottom polystyrene tube (Corning) and incubated on ice for 30 min in the dark with homogenization buffer (250 mM MES; 1% SDS; 1% Triton X-100) adjusted to pH 6 to protect against artificial oxidation during homogenization, as was used in a previous study of lung tissue [42]. ..

    Article Title: The role of Box A of HMGB1 in producing γH2AX associated DNA breaks in lung cancer
    Article Snippet: Cells were then treated with 0.5% RNase (Sigma-Aldrich, St. Louis, MO) for 30 min and 30 μg/mL propidium iodide (PI) solution (BioLegend, San Diego, CA) and incubated at 4 °C for 30 min in dark condition. .. After incubation, the cells were wash with PBS and transfer the sample into 5 ml round bottom polystyrene tube for flow cytometry (FACs tube, Falcon ® Corning). .. The fluorescent signal was detected using the PE channel by BD FACSymphonyTM A1 flow cytometer (Becton Dickinson, Co., Ltd.).

    Article Title: The role of Box A of HMGB1 in producing γH2AX associated DNA breaks in lung cancer.
    Article Snippet: Cells were then treated with 0.5% RNase (Sigma-Aldrich, St. Louis, MO) for 30 min and 30 μg/mL propidium iodide (PI) solution (BioLegend, San Diego, CA) and incubated at 4 °C for 30 min in dark condition. .. After incubation, the cells were wash with PBS and transfer the sample into 5 ml round bottom polystyrene tube for flow cytometry (FACs tube, Falcon® Corning). .. The fluorescent signal was detected using the PE channel by BD FACSymphonyTM A1 flow cytometer (Becton Dickinson, Co., Ltd.).

    Article Title: Improving retinal vascular endothelial cell tropism through rational rAAV capsid design
    Article Snippet: After 96 hours, transduced BMVEC cells were dissociated with TrypLE Express (Gibco™, Dublin, Ireland) into single cells and washed with PBS containing 10% fetal bovine serum ( FBS ) (Gibco™, Dublin, Ireland) and centrifuged at 200xg for 5 minutes. .. The pelleted cells were blocked with 0.5 ml incubation buffer (0.5% BSA, PBS) for 15 minutes at room temperature in a 5 mL round bottom polystyrene tube (Corning, Tewksbury, MA, USA). .. Next, Cells were rinsed in PBS containing 10% FBS and centrifuged at 200xg for 5 minutes prior to labeling them with the anti-mouse CD31 monoclonal primary antibody (5ug/1x10 6 cells, Invitrogen, Carlsbad, CA, USA) in incubation buffer for 90 minutes at room temperature ( RT ).

    Homogenization:

    Article Title: Regulation of hyperoxia-induced neonatal lung injury via post-translational cysteine redox modifications.
    Article Snippet: .. The minced tissue of each sample was collected into their own 5 mL round bottom polystyrene tube (Corning) and incubated on ice for 30 min in the dark with homogenization buffer (250 mM MES; 1% SDS; 1% Triton X-100) adjusted to pH 6 to protect against artificial oxidation during homogenization, as was used in a previous study of lung tissue [42]. ..

    other:

    Article Title: Local irradiation of patient-derived tumors in immunodeficient mice.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-EpCAM Abcam ab223582 Goat anti-rabbit IgG (FITC) 1:300 Abcam ab6717 Phospho-Histone H2AX (Ser139) (20E3) Rabbit mAb 1:300 Cell Signaling 9718S Biological samples Patient-derived esophageal cancer tissue (male, 38y) OR/grossing room N/A Chemicals, peptides, and recombinant proteins Advanced DMEM F/12 Gibco 12634010 Liberase Sigma 05401020001 N2 supplement Invitrogen 17502048 D-Glucose 45% Sigma 50-99-7 Trace elements B Fisher Scientific 25-022 CI Trace elements C Fisher Scientific 25-023-CI HEPES 1 M Thermo Fisher 15630080 Heparin Sigma H3149 Insulin Sigma I9278-5ML b-Mercaptoethanol Sigma 60-24-2 L-Glutamine Gibco 15410314 Penicillin-Streptomycin Thermo Fisher 15140122 Fetal bovine serum (FBS) Gibco 10270106 Phosphate-buffered saline (PBS) tablets Sigma 524650 DNase Sigma 10104159001 Fixation/Permeabilization Kit: Cytoperm and Permwash BD Bioscience 554714 Propium-iodide Sigma-Aldrich 81845 Isoflurane Ecuphar ISOFLO Virkon Fisher Scientific 70693-62-8 Matrigel Corning 356235 Recovery cell culture freezing medium Thermo Fisher 12648010 Eosin Klinipath 3871-2500 Hematoxylin Klinipath 4085-9002 Experimental models: Cell lines Esophageal adenocarcinoma 081R primary line, passage number <10 AMC AMC_EAC_081R Cervical carcinoma SiHa cell line, passage number <10 ATCC HTB-35 Experimental models: Organisms/strains NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG) mice 8 weeks–6 months, males and females Own breeding N/A Nude Hsd:Athymic Nude-Foxn1nu mice 8 weeks–6 months, males and females Envigo 069 Software and algorithms BD FACSDiva software BD Biosciences N/A FlowJo Tree Star version 10.2 https://www.flowjo. com/solutions/flowjo/downloads ImageJ Schneider et al.7 imagej.nih.gov (Continued on next page) STAR Protocols 4, 102098, March 17, 2023 5 Continued REAGENT or RESOURCE SOURCE IDENTIFIER Other 5 mL round bottom polystyrene tube Corning 352052 Cell culture flask T25 Thermo Fisher 169900 Microscope DM IL LED Leica 11521266-B FACS cell sorter Sony SH800 CoolCell LX Cell Freezing Container Corning CLS432001 Radiation source XStrahl RS320 Syringe Luer-Lok 1 mL BD 309628 Needle Orange 25G BD 1712024 Digital caliper Thermo Fisher Scientific 14-648-17 Deposited data Mouse restraining and shielding setup diagrams University of Amsterdam / Trilobes, Joure, the Netherlands Mendeley data: https://doi.org/10.

    Flow Cytometry:

    Article Title: Interactions of graphene oxide and few-layer graphene with the blood-brain barrier
    Article Snippet: .. The pellet was resuspended in the remaining PBS and transferred to a round bottom polystyrene tube (Falcon, Corning) for flow cytometry analysis. ..

    Article Title: The role of Box A of HMGB1 in producing γH2AX associated DNA breaks in lung cancer
    Article Snippet: Cells were then treated with 0.5% RNase (Sigma-Aldrich, St. Louis, MO) for 30 min and 30 μg/mL propidium iodide (PI) solution (BioLegend, San Diego, CA) and incubated at 4 °C for 30 min in dark condition. .. After incubation, the cells were wash with PBS and transfer the sample into 5 ml round bottom polystyrene tube for flow cytometry (FACs tube, Falcon ® Corning). .. The fluorescent signal was detected using the PE channel by BD FACSymphonyTM A1 flow cytometer (Becton Dickinson, Co., Ltd.).

    Article Title: The role of Box A of HMGB1 in producing γH2AX associated DNA breaks in lung cancer.
    Article Snippet: Cells were then treated with 0.5% RNase (Sigma-Aldrich, St. Louis, MO) for 30 min and 30 μg/mL propidium iodide (PI) solution (BioLegend, San Diego, CA) and incubated at 4 °C for 30 min in dark condition. .. After incubation, the cells were wash with PBS and transfer the sample into 5 ml round bottom polystyrene tube for flow cytometry (FACs tube, Falcon® Corning). .. The fluorescent signal was detected using the PE channel by BD FACSymphonyTM A1 flow cytometer (Becton Dickinson, Co., Ltd.).



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